Many studies have examined the impact of cell/embryo culture media on the development of human embryo during IVF process but few studies have followed up and compared the effects of these culture media on the developmental outcome of children conceived by IVF As recurrent experimental evidence fro Jun 21 2014Dimethyl sulfoxide (DMSO) is used extensively as a permeable cryoprotectant and is a common solvent utilized for several water-insoluble substances DMSO has various biological and pharmacological activities however the effect of DMSO on mouse oocyte meiotic maturation remains unknown In DMSO-treated oocytes we observed abnormal MII oocytes that

Cell Culture Protocol 2: Thawing of Frozen Cell Lines

Some cryoprotectants such as DMSO are toxic above 4C therefore it is essential that cultures are thawed quickly and diluted in culture medium to minimize the toxic effects Materials Cell Culture Media : pre-warmed to the appropriate temperature (refer to the ECACC cell line data sheet for the correct medium and temperature )

A major cause of tissue culture medium deterioration is exposure to room fluorescent light Riboflavin and tryptophan present in Dulbecco's modified Eagle's minimum essential medium when exposed to light yield toxic photoproducts responsible for loss of the ability of the medium to support clonal growth of human mouse and Chinese hamster cell lines

Media that inhibit the growth of unwanted microorganisms and support the growth of the organism of interest by supplying nutrients and reducing competition are called selective media An example of a selective medium is MacConkey agar It contains bile salts and crystal violet which interfere with the growth of many gram-positive bacteria and favor the growth of gram-negative bacteria

Jul 17 2014An effect of culture media on embryo quality after ICSI from the second day of culture was also demonstrated by Cossiello et al The exact composition of the compared media is not disclosed so specific components cannot be linked to the outcome

The results presented in Table 1 show that the oxygen solubility in the culture medium before inoculation of microorganism was 6 772 mgO 2 L −1 11 8% lower when compared to the oxygen solubility in distilled water (7 677 mgO 2 L −1) Saturation percentageversus dissolved oxygen data was highly linear as shown in Figures 2 and 3 (𝑅 2 0 9 9) At the other hand the oxygen

Peptidomic analysis of blastocyst culture medium and the

Peptidomic analysis of blastocyst culture medium and the effect of peptide derived from blastocyst culture medium on blastocyst formation and viability Hui Ji High‐quality in vitro human embryo culture medium can improve the blastocyst formation rate and blastocyst quality and be beneficial for the clinical application of single

Effect of different culture media on rnvcelial growth A Oyster mushroom culture grovvng in malt agar medium a Oyster mushroom culture growing in MS medium C D Miniature mushrooms developing from the over ripe cultures of Pleurorus osrrearus van sajar caju in malt extract agar medium

The beneficial effects of using combined permeating and nonpermeating cryoprotectants in this study concur with previous reports in the literature that showed combinations of 18% raffinose and 1 75% glycerol (Tada et al 1990) 10% raffinose and 5% glycerol (Yokoyama et al 1990) and 6% glycerol plus trehalose (Storey et al 1998) provided for

Effect of culture medium and pH on mycelia growth The mycelium growth in different solid nutritive culture media (MMN or PDA) and at different pH val-ues (5 or 6) is shown in Table I In general the myceli-um growth varied either with the culture medium or pH medium For Lactarius deliciosus MMN proved to be a better culture medium than PDA

It is shown that if cystine is used instead of cysteine in the cell culture medium coloration was reduced Hydrocortisone has been shown to reduce coloration and boost specific productivity The effect of a peptone/hydrolysate on coloration was investigated in cell culture experiments which showed its use can lead to reduced coloration

The addition ofN-2-hydroxyethylpiperazine-N′-2-ethanesulfonic acid (HEPES) to RPMI 1640 medium markedly increases the production of cytotoxic products during exposure of the medium to visible light The cytotoxicity has been analyzed by measuring uptake of [3H]thymidine by murine thymocytes cultured in preirradiated medium containing 25 mM HEPES

Effect of Sucrose on Culture Medium on Shoot and Root Growth Explants of purple coneflower shoots (1 cm) were introduced to the MS basal medium containing 0 3 mgL −1 BA and 0 01 mgL −1 NAA and three concentrations of sucrose (2% 3% and 4%) to assess the shoot growth and root development

The beneficial effects of using combined permeating and nonpermeating cryoprotectants in this study concur with previous reports in the literature that showed combinations of 18% raffinose and 1 75% glycerol (Tada et al 1990) 10% raffinose and 5% glycerol (Yokoyama et al 1990) and 6% glycerol plus trehalose (Storey et al 1998) provided for

Effect of cell culture medium components on color of

Jun 27 2013This manuscript summarizes cell culture experiments and reports on findings regarding the effect of various media components that contribute to drug substance color for a specific monoclonal antibody In this work lower drug substance color was achieved via optimization of the cell culture medium

Growth medium also called Culture Medium or Nutrient Broth solution freed of all microorganisms by sterilization (usually in an autoclave where it undergoes heating under pressure for a specific time) and containing the substances required for the growth of microorganisms such as bacteria protozoans algae and fungi The medium may be solidified

Growth medium also called Culture Medium or Nutrient Broth solution freed of all microorganisms by sterilization (usually in an autoclave where it undergoes heating under pressure for a specific time) and containing the substances required for the growth of microorganisms such as bacteria protozoans algae and fungi The medium may be solidified

Introduction The growth of microorganisms in an artificial medium is influenced by several physical and chemical factors A nutrient material prepared for the growth of microorganisms in a laboratory is called culture medium and the nutrient composition of a culture medium plays a major role in microbial growth (Toratora et al ) 27 In laboratory fungi are isolated on specific

Effect of culture medium and cryoprotectants on the growth and survival of probiotic lactobacilli during freeze drying Siaterlis A(1) Deepika G Charalampopoulos D Author information: (1)Department of Food Biosciences The University of

Effect of different culture media on rnvcelial growth A Oyster mushroom culture grovvng in malt agar medium a Oyster mushroom culture growing in MS medium C D Miniature mushrooms developing from the over ripe cultures of Pleurorus osrrearus van sajar caju in malt extract agar medium environment example Morchej/a spp (e dible

Nannochloropsis salina is a marine free living nanoplanktonic alga with biotechnological potential Among the three Common algal culture media studied Guillard's f/2 medium produced better biomass growth rate and production of Chl a when compared to Walne's and Chu#10 media There was not much difference among the three media when it came to the amount of

TAKAYAMA S M MISAWA K KO T MISATO 1977 Effect of cultural conditions on the growth of Agrostemma githago cells in suspension culture and the concomitant production of an anti-plant virus substance Physiol Plant 41: 313–320 CrossRef Google Scholar

The culture medium must provide mineral and organic nutrients the balance of growth regulators should decrease cytokinins and increase exogenous auxin Agave sp shoots were rooted in a growth regulator-free MS medium (Zhang et al 2013) The combination of auxins and reduced MS salt concentration in the culture media have a promontory effect on