Metabolites are low molecular weight compounds involved in the chemical reactions taking place in the cells of living organisms to uphold life i e metabolism The research field of metabolomics investigates the relationship between metabolite alterations and biochemical mechanisms e g disease processes D-optimal design of an untargeted HS-SPME-GC-TOF metabolite profiling method Bruno Fedrizzi a Silvia Carlin a One of the youngest is undoubtedly metabolomics which aims to define the whole chemical fingerprint unique to each specific organism The development and optimisation of an untargeted high-throughput method capable of investigating

Quantitative 1H Nuclear Magnetic Resonance Metabolite

Targeted Metabolite Profiling Metabolite identification and quantification were achieved using the Profiler feature of Chenomx NMR Suite version 4 6 for analysis of one-dimensional 1 H NMR spectra Chenomx Profiler is linked to a database of metabolites

Nov 12 2018Chemical fingerprinting of secondary metabolites in biological samples requires a high spectral selectivity and sophisticated data evaluation In complex biological material this is even more complicated due to the autofluorescence of the sample matrix which however can also be used to get a more complete picture of the sample

Feb 20 2015Metabolite profiling is critical in many aspects of the life sciences particularly natural product research Obtaining precise information on the chemical composition of complex natural extracts (metabolomes) that are primarily obtained from plants or microorganisms is a challenging task that requires sophisticated advanced analytical methods

Comparative metabolite profiling and fingerprinting of medicinal licorice roots using a multiplex approach of GC-MS LC-MS and 1D NMR techniques Farag MA(1) Porzel A Wessjohann LA Author information: (1)Department of Bioorganic Chemistry Leibniz Institute of Plant Biochemistry Halle (Saale) Germany

Chemical methods for the enhancement of residual blood fingerprints have been successfully used for years Leucomalachite green amido black and ninhydrin chemically react with components in blood to form a dark-colored dye complex and have all been used successfully on light-colored or transparent surfaces

Recommendations for Reporting Metabolite Data

However to date even the most comprehensive methods detect only between 1000 and 2000 molecular features which are putatively indicative of bona fide chemical entities synthesized in plant tissues (Aharoni et al 2002 Giavalisco et al 2009 Iijima et al 2008) Metabolite measurements are further complicated by the chemical

Comparative metabolite profiling and fingerprinting of medicinal licorice roots using a multiplex approach of GC-MS LC-MS and 1D NMR techniques Farag MA(1) Porzel A Wessjohann LA Author information: (1)Department of Bioorganic Chemistry Leibniz Institute of Plant Biochemistry Halle (Saale) Germany

To rapidly clarify and quantify the chemical profiling of Cinnamomi cortex a reliable and feasible strategy of chromatographic fingerprinting with a suite of chemometrics methods was developed and validated by ultra-high performance liquid chromatography coupled with diode array detection Furthermore to identify more meaningful chemical markers the chemometrics methods

Aug 16 2017Methods for discovering metabolomes range from technical chemical methods such as NMR to statistical analysis The Sumner Group The Sumner Group is involved in the research of plant metabolomics This is challenging because of 3 primary limitations Profiling the metabolome is difficult because of its chemical

Sep 04 2015Metabolic foot- and fingerprinting is defined as the semi-quantitative analysis of extracellular (exo-metabolome) and intracellular (the endo-metabolome) metabolites respectively [] The main source of variability in the metabolic footprint is the presence of living cells in the medium which are responsible for metabolite uptake and secretion and secretion

Profiling also known as differential expression this step involves finding metabolites with statistically significant variations within control and test sample sets Compound identification is the determination of the chemical structure of the discovered metabolites

Metabolites are low molecular weight compounds involved in the chemical reactions taking place in the cells of living organisms to uphold life i e metabolism The research field of metabolomics investigates the relationship between metabolite

Gas chromatography–mass spectrometry (GC‐MS)–based metabolomics is ideal for identifying and quantitating small‐molecule metabolites (650 Da) including small acids alcohols hydroxyl acids amino acids sugars fatty acids sterols catecholamines drugs and toxins often using chemical derivatization to make these compounds sufficiently volatile for gas chromatography

Chemoselective probes for metabolite enrichment and profiling

T1 - Chemoselective probes for metabolite enrichment and profiling AU - Carlson Erin E AU - Cravatt Benjamin F PY - 2007/5/1 Y1 - 2007/5/1 N2 - Chemical probes that target classes of proteins based on shared functional properties have emerged as powerful tools for proteomics

Profiling also known as differential expression this step involves finding metabolites with statistically significant variations within control and test sample sets Compound identification is the determination of the chemical structure of the discovered metabolites

Metabolite profiling of representative samples for each of the four clusters identified 50 compounds: amino acids saccharides phenolic acids flavonoids and lipids In general the saccharides and lipids were primarily responsible for discriminating between the two genetic lines and ambient and elevated CO2

Sep 04 2015Metabolic foot- and fingerprinting is defined as the semi-quantitative analysis of extracellular (exo-metabolome) and intracellular (the endo-metabolome) metabolites respectively [] The main source of variability in the metabolic footprint is the presence of living cells in the medium which are responsible for metabolite

Metabolic fingerprinting refers to the use of machine output as potentially recognizable chemical pattern specific of an individual sample Metabolite fingerprinting by NMR is a fast convenient and effective tool for discriminating between groups of related samples and it identifies the most important regions of the spectra for further analysis

2 Investigating metabolites in mushroom tissues using GC/MS as a metabolic profiling technique The method was used to profile mushroom samples to identify metabolic markers for damage and to gain understanding of the many metabolic processes that occur 3

The answer may lie with metabolomics which is the study of the unique chemical fingerprints that specific cellular processes—processes like cancer—leave behind While mRNA gene expression data and proteomic analyses do not tell the whole story of what might be happening in a cell metabolite profiling can give an instantaneous snapshot

Apr 25 2016Farag MA Porzel A Wessjohann LA Comparative metabolite profiling and fingerprinting of medicinal licorice roots using a multiplex approach of GC–MS LC–MS and 1D NMR techniques Phytochemistry 2012 76:60–72 CAS Article PubMed Google Scholar