ABSTRACT: Buffer salts are often added to culture medium to promote bacterial growth However we found that buffer salts can improve the freeze-drying survival rate In this experiment the mechanisms for the effects of different buffer salts on the survival rate of freeze-dried strains were examined The results showed that buffer salts had Yeast is that wonderful microbe which converts sweet wort into an enjoyable alcoholic beverage In addition to converting sugar to alcohol yeast can also influence the taste flavor bouquet and even the color of beer They do this by secreting a variety of compounds at very low levels Different yeast strains produce different levels of these compounds and therefore impart their own subtle

Processing and storage effects on stability of

There is increasing use of dietary supplements and functional foods to help reduce future health care costs or provide supplemental nutrition The stability of the actives or loss of bioavailability of the protein in these products during processing and storage of either dry or semi-moist based products is a key to quality and required by GMPs

Effect of NaHCO3 MgSO4 sodium ascorbate sodium glutamate phosphate buffer on survival of lactobacillus bulgaricus during freeze-drying Advance Journal of Food Science and Technology 5(6) 771-774 Cushman D W Cheung H S (1971) Spectrophotometric assay and properties of the angiotensin-converting enzyme of rabbit lung

before freeze-drying and cell viability determined after freeze-drying and 8-week of cold storage 2 3 Enumeration of encapsulated L casei For quantitative determination of cell viability it was necessary to release the encapsulated L casei from the particles Thus one gram of particles was suspended in 9 ml phosphate buffer solution

06/08/2015The method for preserving a mammalian embryo(s) or fertilized egg(s) of the present invention comprises immersing a mammalian embryo(s) or fertilized egg(s) in a medium containing 20 to 80% (v/v) serum and 10 to 100 mM Good's buffer and storing the embryo(s) or fertilized egg(s) at non-freezing low temperature The preservative solution for a mammalian embryo(s) or fertilized egg(s) of

19/08/2004In order to evaluate the survival of lactobacilli in acidic conditions we compared the survival of five Lactobacillus strains in simulated gastric juice pH 2 0 for 90 min L rhamnosus GG had the highest survival rate over the 90 min of exposure to simulated gastric juice (pH 2 0) while the poorest survivor was Lactobacillus paracasei NFBC 338 whose concentration declined to undetectable


US3261761A US193027A US19302762A US3261761A US 3261761 A US3261761 A US 3261761A US 193027 A US193027 A US 193027A US 19302762 A US19302762 A US 19302762A US 3261761 A US3261761 A US 3261761A Authority US United States Prior art keywords culture temperature freeze drying moisture sublimation Prior art date 1962-05-07 Legal status (The legal status is an assumption

Effects on numbers of larger aberrant crypt foci suggested synergistic activity compared with the individual prebiotic and probiotic treatments alone (Rowland 1998) Additive effects were found in another study showing that administration of B longum and oligofructose resulted in reduced numbers of aberrant crypts in rats given the carcinogen 1 2-dimethylhydrazine (Gallaher Khil 1999) In

The shear rate to which probiotics are exposed during atomization is arguably a parameter decreasing viability (Riveros et al 2009 Santivarangkna et al 2008) but a recent study (Ghandi et al 2012) showed that the survival of Lactococcus lactis subsp cremoris was greatly diminished by an increased shear rate This study also demonstrates that damage caused by oxygen exposure resulted in

As observed in treatments with exogenous trehalose survival curves showed an initial rapid decline in viability followed by slower linear decreases although both the extent of the initial decline and the subsequent rate of decrease were reduced at the higher intracellular trehalose concentration with final cell viabilities of 2 210 3 and 6 510 5 CFU ml −1 equivalent to 0 002 and 2 1

Water is an inorganic transparent tasteless odorless and nearly colorless chemical substance which is the main constituent of Earth's hydrosphere and the fluids of all known living organisms It is vital for all known forms of life even though it provides no calories or organic nutrients Its chemical formula is H 2 O meaning that each of its molecules contains one oxygen and two hydrogen

XLIX Salinity effects on survival maturity growth biometrics reproductive and lifespan characteristics of a bisexual and a parthenogenetic population of Artemia Hydrobiologia 302:215-227 Vanhaecke P and Sorgeloos P 1982 International Study on Artemia XVIII The hatching rate of Artemia cysts - a comparative study Aquacultural Eng

The beneficial effects of coffee have been studied heavily in the last few decades Coffee has been found to prevent numerous degenerative diseases and even some forms of cancer There have been studies on how coffee can decrease the oxidative damage on white blood cells but this is the first study showing it to reduce the damage done to strands of DNA As the scientific body of evidence

14/04/2014In addition to mechanically damaging cells ice crystals can also cause the salts and proteins in the buffer to become concentrated This problem is known as freeze concentration and can cause significant stress on the stability of proteins Although the exact mechanism of ice-induced protein denaturation is not fully understood we do know that changes in the physical environment of the

Optimization of Protective Agents for The Freeze

The effects of several selected protective agents on the viability of P polymyxa Kp10 after freeze-drying were studied Response surface methodology (RSM) was used for optimizing formulation for the protective agents The combination of lactose (10% w/v) skim milk (20% w/v) and sucrose (27 5% w/v) was found to be suitable for preserving P polymyxa Kp10 during freeze-drying Further P

Water (H2 O) is a polar inorganic compound that is at room temperature a tasteless and odorless liquid nearly colorless with a hint of blue This simplest hydrogen chalcogenide is by far the most studied chemical compound and is described as the universal solvent for its ability to dissolve many substances This allows it to be the solvent of life: indeed water as found in nature almost

All manipulations were performed at room temperature The survival rate was calculated as cells/ml after drying with reference to cells/ml before drying expressed as a percentage The assays were performed in triplicate Sporulation test To determine if the isolated strains were sporulant a 4-day-old colony was resuspended in 1 ml of sterile saline M9 buffer and incubated at 72C for 30

The rate of reactivation depends on the structure of the phosphoryl moiety bound to the enzyme the source of the enzyme the structure and concentration of oxime which is present at the active site and the rate of post-inhibitory dealkylation known as aging Phosphorylated oximes are formed during reactivation reaction and some of the appear to be potent inhibitors of /acetylcholinesterase

09/03/2020Freeze drying of Lactobacillus plantarum GP in the presence of wall materials to achieve improved survival and retention of probiotic functionality during storage L plantarum cells were lyophilized in the presence of inulin fructooligosaccharides lactulose and/or skim milk The lyophilized vials were stored at 8–10 C up to 6 months and cells from these vials were evaluated for their

Freeze-drying is often the method of choice but other methods such as by vacuum or under a stream of nitrogen can be used The vesicles are then rehydrated [7-74] A mechanism has been proposed whereby as the vesicles become more concentrated during dehydration they flatten and fuse forming multilamellar planes where the solute is sandwiched Therefore on hydration larger vesicles are

Effect of NaHCO3 MgSO4 sodium ascorbate sodium glutamate phosphate buffer on survival of lactobacillus bulgaricus during freeze-drying Advance Journal of Food Science and Technology 5(6) 771-774 Cushman D W Cheung H S (1971) Spectrophotometric assay and properties of the angiotensin-converting enzyme of rabbit lung